产品货号：
GS2206
中文名称：
BstV1I内切酶
英文名称：
BstV1I
产品规格：
50U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
At 37℃ activity is 10%from maximum.
技术规格
相关搜索：BstV1I内切酶，BstV1I限制性内切酶
使用说明
At 37℃ activity is 10%from maximum.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof pBR322 DNA in 1hourat 55℃ in a total reaction volume of 50μl. |
| Recognition sites | GCAGC(N)8↑ |
| 来源 | Bacillus stearothermophilus V1 |
| Assayed on | pBR322 DNA |
| Optimal buffer | G?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.) |
| Optimal temperature | 55℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);200 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof DNA with 2 u.a.of enzyme for 16hoursat 55℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BbvI,BseXI,Bst71I,R9896 |
相关搜索：BstV1I内切酶，BstV1I限制性内切酶